Thermo Fisher Scientific Collagen I Monoclonal Antibody (5D8-G9)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:10 - 1:100

-

Immunohistochemistry (Frozen) (IHC (F))

1:200-1:1,000

-

ELISA (ELISA)

0.4-50 µg/mL

-

Product Specifications

Species Reactivity

Bovine, Human, Pig

Published species

Human, Pig

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5D8-G9

Immunogen

Purified, full-length native human collagen I protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Collagen I,uniProtId:P02452-1,ncbiNodeId:9606,antigenRange:1-1464,antigenLength:1464,antigenImageFileName:MA1-141_Collagen_I_P02452-1_House_mouse.svg,antigenImageFileNamePDP:MA1-141_Collagen_I_P02452-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536845

Product Specific Information

MA1-141 detects collagen I from bovine, human, and porcine. This antibody will not cross react with collagen 2-11, or detect thermally denatured collagen. Western blot analysis of recombinant bovine collagen I using MA1-141 detected a predominant band around 270kD likely corresponding to the alpha-1 dimer and a fainter band at a higher MW likely corresponding to the alpha-1/alpha-2 trimer.

MA1-141 has been successfully used in ELISA, immunofluorescence, immunohistochemistry (frozen and paraffin), and Western blot procedures. The epitope is sensitive to routine formalin fixation and paraffin embedding. Strong staining of connective tissue fibres is seen in acetone-fixed or unfixed frozen sections.

Target Information

Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity. Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.