Thermo Fisher Scientific DYKDDDDK Tag Monoclonal Antibody (L5)
DYKDDDDK(FALG) 태그 단백질 검출용 Rat 모노클로날 항체(L5 클론). Western blot, ELISA, ICC/IF 등 다양한 응용에 적합. 비결합형 액상 포맷, Protein G 정제, 1 mg/mL 농도. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 10 publications |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| ELISA | 100 ng/mL | - |
| Immunoprecipitation (IP) | - | View 1 publication |
| Functional Assay | - | View 1 publication |
| Miscellaneous (PubMed) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host/Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | L5 |
| Immunogen | Recombinant fusion proteins of mouse Langerin/CD207 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536846 |
Available Conjugated Formats
Product Specific Information
The MA1-142 antibody (clone L5) has been successfully used as a detection antibody in direct ELISA with FLAG-tagged protein-coated plates.
It recognizes a similar epitope as the FLAG tag from Sigma.
The immunogen is a recombinant fusion protein of mouse Langerin/CD207 containing a flexible linker sequence from E. coli OmpF and a FLAG epitope (J Immunol Methods. 2008 Feb 29;331(1-2):27-38. Epub 2007 Nov 26).
Target Information
DYKDDDDK tag (FLAG tag) is a short peptide sequence widely used as a protein tag for purification, detection, and localization.
It consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, and is often fused to the N- or C-terminus of a protein of interest.
The tag enables easy identification and isolation of proteins using anti-FLAG antibodies.
DYKDDDDK tag is more hydrophilic than other common epitope tags, minimizing the risk of protein denaturation or inactivation.
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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