Thermo Fisher Scientific Grim-19 Monoclonal Antibody (1A8), eBioscience
Grim-19 단백질을 인식하는 1A8 클론의 단클론 항체로, 인간·마우스·랫트에 반응합니다. WB, IHC, ICC 등 다양한 응용에 사용 가능하며, 0.5 mg/mL 농도의 액상 형태입니다. 미토콘드리아 복합체 I 구성요소 연구에 적합합니다.
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Thermo Fisher Scientific Grim-19 Monoclonal Antibody (1A8), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 5 µg/mL | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent | - |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | Assay-Dependent | - |
| Flow Cytometry (Flow) | - | View 5 publications |
| In vitro Assay (IV) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1A8 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10597591 |
Product Specific Information
This 1A8 monoclonal antibody reacts with human, mouse, and rat Grim-19. Grim-19 (16 kDa) is expressed in many human tissues, especially in the heart and skeletal muscle, with lower levels in liver, kidney, and brain. Initially described as a nuclear protein, Grim-19 is primarily localized to mitochondria as an essential component of complex I of the electron transport chain. Its expression can be induced by interferon-beta and all-trans-retinoic acid in tumor cell lines, leading to cell death. Grim-19 binds the transactivation domain of STAT3 and inhibits its transcriptional activity.
Applications Reported: Immunoblotting, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections, and immunocytochemistry.
Applications Tested: Tested by immunoblotting of HeLa cell lysate at ≤5 µg/mL. Antibody should be titrated for optimal performance.
Purity: >90% (SDS-PAGE).
Aggregation: <10% (HPLC).
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
GRIM-19 (Gene associated with Retinoid-IFN-induced Mortality) is a novel gene identified to regulate cell death. Antisense expression of GRIM-19 confers resistance to IFN/RA-induced death by reducing intracellular GRIM-19 protein levels, while overexpression enhances cell death in response to IFN/RA. GRIM-19 is primarily nuclear, and its expression is induced by IFN/RA combination, indicating it functions as a cell death-regulatory molecule.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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