Thermo Fisher Scientific Grim-19 Monoclonal Antibody (1A8), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

5 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

-

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-Dependent

-

Flow Cytometry (Flow)

-

View 5 publications 5 publications

In vitro Assay (IV)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

1A8

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10597591

Product Specific Information

Description: This 1A8 monoclonal antibody reacts with human, mouse, and rat Grim-19. This 16 kDa protein is expressed in many human tissues, especially in the heart and skeletal muscle. Lower expression levels of Grim-19 can be observed in the liver, kidney, and brain. Originally described as a nuclear protein, reports indicate that Grim-19 is primarily localized to mitochondria as an essential component of complex I of the electron transport chain. Grim-19 expression can be induced by interferon-beta and all-trans-retinoic acid in tumor cell lines, leading to cell death. Finally, Grim-19 has been shown to bind the transactivation domain of STAT3 and inhibit its transcriptional activity. The molecular mechanisms of how Grim-19 regulates STAT3 within the cell remains under active investigation.

Applications Reported: This 1A8 antibody has been reported for use in immunoblotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.

Applications Tested: This 1A8 antibody has been tested by immunoblotting of HeLa cell lysate. This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

A novel gene associated with Retinoid-IFN-induced Mortality (GRIM) GRIM-19 gene was identified. Antisense expression of GRIM-19 confers a strong resistance against IFN/RA-induced death by reducing the intracellular levels of GRIM-19 protein. Overexpression of GRIM-19 enhances cell death in response to IFN/RA. GRIM-19 is primarily a nuclear protein whose expression is induced by the IFN/RA combination. These data indicate that GRIM-19 is a novel cell death-regulatory molecule.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.