Thermo Fisher Scientific Caspase 11 Monoclonal Antibody (17D9), eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

Description:
The monoclonal antibody 17D9 recognizes mouse Caspase-11, a cysteine protease involved in apoptosis and inflammation. Caspase-11 exists as 43 kDa and 38 kDa pro-caspase forms and 30 and 10 kDa active forms. The 17D9 antibody recognizes p43, p38, and p30 forms. Caspase-11 activates other caspases such as caspase-3, -7, and -1, which in turn activate IL-1β.
Under Caspase-11-deficient conditions, cells fail to process or secrete IL-1β and exhibit motility defects due to disruption of interaction with Aip1, an actin-binding protein. Caspase-11 is homologous (~60%) to human Caspase-4 and is upregulated by LPS treatment in macrophages, lymphocytes, and hepatocytes.

Applications Reported:
This 17D9 antibody has been reported for use in immunoblotting (WB).

Applications Tested:
Tested by western blotting at 1–5 µg/mL on J774 cells or stimulated splenocyte lysates. Samples must be reduced for detection. It is recommended to titrate the antibody for optimal performance.

Purity: Greater than 90%, determined by SDS-PAGE.
Aggregation: Less than 10%, determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene encodes a cysteine-aspartic acid protease (caspase) family member. Sequential activation of caspases is central to the execution phase of apoptosis. Caspases exist as inactive proenzymes requiring proteolytic processing at conserved aspartic residues to generate active heterodimeric enzymes. This caspase can cleave and activate its own precursor and caspase-1 precursor. Overexpression induces apoptosis. Alternative splicing results in distinct isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.