Thermo Fisher Scientific RANGAP1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 398-587 of human RanGAP1 (NP_0028741) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RANGAP1,uniProtId:P46060-1,ncbiNodeId:9606,antigenRange:398-587,antigenLength:587,antigenImageFileName:PA5-92435_RANGAP1_P46060-1_Rabbit.svg,antigenImageFileNamePDP:PA5-92435_RANGAP1_P46060-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.54 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2806498

Product Specific Information

Immunogen sequence: PQQRGQGEKS ATPSRKILDP NTGEPAPVLS SPPPADVSTF LAFPSPEKLL RLGPKSSVLI AQQTDTSDPE KVVSAFLKVS SVFKDEATVR MAVQDAVDAL MQKAFNSSSF NSNTFLTRLL VHMGLLKSED KVKAIANLYG PLMALNHMVQ QDYFPKALAP LLLAFVTKPN SALESCSFAR HSLLQTLYKV; Positive Samples: BT-474, HeLa, MCF7, A-549, Jurkat; Cellular Location: Chromosome, Cytoplasm, Cytoplasmic side, Nucleus membrane, Peripheral membrane protein, centromere, cytoskeleton, kinetochore, spindle pole

Target Information

Ran is a small signaling GTPase that is involved in nucleocytoplasmic transport. Two additional functions of animal Ran in the formation of spindle asters and the reassembly of the nuclear envelope in mitotic cells have been recently reported. In contrast to Ras or Rho, Ran is not associated with membranes. Instead, the spatial sequestering of its accessory proteins, the Ran GTPase-activating protein RanGAP and the nucleotide exchange factor RCC1, appears to define the local concentration of RanGTP vs. RanGDP involved in signaling. Mammalian RanGAP is bound to the nuclear pore by a mechanism involving the attachment of small ubiquitin-related modifier protein (SUMO) to its C terminus and the subsequent binding of the SUMOylated domain to the nucleoporin Nup358.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.