Takara Lentiviral vector systems for constitutive gene expression

These lentiviral expression systems are designed for constitutive gene expression from either a CMV or EF1-alpha promoter.

These lentiviral expression systems are designed for constitutive gene expression from either a CMV or EF1-alpha promoter.

Biscistronic options

The IRES Vectors included with the bicistronic lentiviral expression systems each contain a multiple cloning site located upstream of the IRES from which a selection marker is expressed. Because your gene of interest and a selection marker are translated from a single RNA, you can be certain that nearly 100% of the colonies that are resistant to puromycin, hygromycin, and G418 also express your protein of interest.

Fluorescent protein options

For lentiviral vectors that express fluorescent protein tags and markers, including the bicistronic pLVX-IRES-ZsGreen1 and pLVX-IRES-mCherry vectors, see our Fluorescent Lentiviral Expression Vectors webpage.

EF1-alpha promoter versions

Lenti-X lentiviral vector systems with EF1-alpha promoters allow you to achieve robust, constitutive, long-term expression of your gene of interest in cell types in which CMV promoters are often silenced, such as hematopoietic and stem cells.

Lentiviral packaging system included

Many of our lentiviral systems are complete systems that include our high-titer Lenti-X Packaging Single Shots (VSV-G) when used with the Lenti-X 293T Cell Line (not included), generates titers that are far superior to those of other commercially available lentiviral packaging systems. Frequently, an entire dish of target cells can be effectively transduced using just 10–100 µl of unconcentrated viral supernatant.

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