Takara RHB-A: neural stem cell culture and differentiation medium

RHB-A is a fully defined, serum-free cell culture medium optimized for the derivation, maintenance, expansion, and differentiation of human and mouse neural stem (NS) cells. RHB-A is specifically formulated for culturing pure populations of adherent human and mouse NS cells.

RHB-A is a fully defined, serum-free cell culture medium optimized for the derivation, maintenance, expansion, and differentiation of human and mouse neural stem (NS) cells. RHB-A is specifically formulated for culturing pure populations of adherent human and mouse NS cells. This medium ensures the long-term stability of NS cell differentiation capacity (Sun et al. 2008) and can be used to support differentiation of human and mouse NS cells into functional neurons (Sun et al. 2008; Conti et al. 2005; Hansen et al. 2010) and of mouse ES cells into neural precursors (Ying et al. 2003; Diogo, Henrique, and Cabral 2008; Abranches et al. 2009). Critically, RHB-A medium is more efficient at neural differentiation than conventional N2- and B27-based methods. RHB-A medium can be used to support organotypic slice culture of central nervous system tissues such as brain and spinal cord, and supports the culture of glioblastoma stem cells used in cancer research.

NOTE: RHB-A medium requires supplementation with Epidermal Growth Factor (EGF) and Fibroblast Growth Factor (FGF-2) for most applications (not supplied). RHB‐Basal medium does not contain any growth factors or neuronal supplements. Therefore, this medium can be tailored to the specific requirements of your cell type by the addition of supplements.

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Overview

• RHB-A is a proprietary, fully defined, and serum-free medium designed to maintain pure populations of adherent human and mouse NS cells

• RHB-Basal medium is free of animal components and neuronal supplements; the medium can be customized by the addition of supplements

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Applications

• Derivation of mouse and human NS cells from ES cells and fetal and adult tissues

• Maintenance and propagation of adherent mouse and human NS cells

• Differentiation of mouse and human NS cells into functional neurons

• Differentiation of mouse ES cells to neuronal precursors

• Organotypic slice culture of CNS tissue

• Glioblastoma stem cell culture for cancer research

• Refer to the Data Sheet for additional examples of use

Components

• 500 ml medium

References

Abranches, E. et al. Neural Differentiation of Embryonic Stem Cells In Vitro: A Road Map to Neurogenesis in the Embryo. PLoS One 4, e6286 (2009).

Conti, L. et al. Niche-Independent Symmetrical Self-Renewal of a Mammalian Tissue Stem Cell. PLoS Biol. 3, e283 (2005).

Diogo, M. M., Henrique, D. & Cabral, J. M. S. Optimization and integration of expansion and neural commitment of mouse embryonic stem cells. Biotechnol. Appl. Biochem. 49, 105 (2008).

Hansen, D. V., Lui, J. H., Parker, P. R. L. & Kriegstein, A. R. Neurogenic radial glia in the outer subventricular zone of human neocortex. Nature 464, 554–561 (2010).

Sun, Y. et al. Long-term tripotent differentiation capacity of human neural stem (NS) cells in adherent culture. Mol. Cell. Neurosci. 38, 245–258 (2008).

Ying, Q. L., Stavridis, M., Griffiths, D., Li, M. & Smith, A. Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture. Nat. Biotechnol. 21, 183–186 (2003).

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.