Thermo Fisher Scientific Cullin 3 Polyclonal Antibody
Western blot, ICC/IF, ELISA에 적합한 Cullin 3 폴리클로날 항체. Human, Mouse, Rat 반응성. Rabbit IgG 기반으로 제작되었으며 액상 형태, 1 mg/mL 농도. Affinity Chromatography로 정제, -20°C 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 468–768 of human Cullin 3 (NP_003581.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2913675 |
Product Specific Information
- Positive test controls: SH-SY5Y, Mouse brain
- Subcellular localization: Golgi apparatus, Nucleus
Immunogen sequence:
SKLEGMFRDM SISNTTMDEF RQHLQATGVS LGGVDLTVRV LTTGYWPTQS ATPKCNIPPA PRHAFEIFRR FYLAKHSGRQ LTLQHHMGSA DLNATFYGPV KKEDGSEVGV GGAQVTGSNT RKHILQVSTF QMTILMLFNN REKYTFEEIQ QETDIPEREL VRALQSLACG KPTQRVLTKE PKSKEIENGH IFTVNDQFTS KLHRVKIQTV AAKQGESDPE RKETRQKVDD DRKHEIEAAI VRIMKSRKKM QHNVLVAEVT QQLKARFLPS PVVIKKRIEG LIEREYLART PEDRKVYTYV A
Target Information
Cullin 3 is a member of the family of human cullin genes (CUL 1, 2, 3, 4a, 4b, and 5) homologous to the S. cerevisiae cdc 53 gene.
CUL 1 forms a complex with human p19 skp1 and F box protein p45 skp2. Together with cdc 53 (known as Cul A), they form a ubiquitin ligase complex controlling proteolysis of G1 cell cycle regulatory proteins.
In contrast, CUL 2 does not associate with p19 skp1 and p45 skp2 but forms a complex with the inactive transcriptional elongation complex SIII, composed of Elongin C, Elongin B, and VHL. The function of this association remains unknown.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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