Thermo Fisher Scientific Cullin 3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 468-768 of human Cullin 3 (NP_0035811) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cullin 3,uniProtId:Q13618-1,ncbiNodeId:9606,antigenRange:468-768,antigenLength:768,antigenImageFileName:PA5-120103_Cullin_3_Q13618-1_Rabbit.svg,antigenImageFileNamePDP:PA5-120103_Cullin_3_Q13618-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2913675

Product Specific Information

Positive test controls include: SH-SY5Y, Mouse brain. The target is usually found in the following locations: Golgi apparatus, Nucleus.

Immunogen sequence: SKLEGMFRDM SISNTTMDEF RQHLQATGVS LGGVDLTVRV LTTGYWPTQS ATPKCNIPPA PRHAFEIFRR FYLAKHSGRQ LTLQHHMGSA DLNATFYGPV KKEDGSEVGV GGAQVTGSNT RKHILQVSTF QMTILMLFNN REKYTFEEIQ QETDIPEREL VRALQSLACG KPTQRVLTKE PKSKEIENGH IFTVNDQFTS KLHRVKIQTV AAKQGESDPE RKETRQKVDD DRKHEIEAAI VRIMKSRKKM QHNVLVAEVT QQLKARFLPS PVVIKKRIEG LIEREYLART PEDRKVYTYV A

Target Information

Cullin 3 is a member of the family of human cullin genes (CUL 1, 2, 3, 4a, 4b, and 5) homologous to the S. cerevisiae cdc 53 gene. CUL 1 forms a complex with human p19 skp1 and F box protein p45 skp2. Together with cdc 53 (Known as Cul A), as three subunits, they form a ubiquitin ligase-controlling proteolysis of G1 cell cycle regulatory proteins. In contrast to CUL 1, CUL 2 does not associate with p19 skp1 and p45 skp2. Instead, CUL 2 forms a complex with an inactive transcriptional elongation complex, SIII, formed by three subunits: Elongain C, Elongin B and VHL. The function of this association remains unknown.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.