Thermo Fisher Scientific Phospho-SMAD2 (Ser250) Polyclonal Antibody
SMAD2 단백질의 Ser250 인산화 형태를 특이적으로 인식하는 rabbit polyclonal 항체. Western blot 및 ICC/IF에 사용 가능하며, 인간, 마우스, 랫트 시료에 반응. 1 mg/mL 농도의 액상 형태로 제공되며, PBS/glycerol buffer에 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human SMAD2 (Accession Q15796), corresponding to amino acid residues around phosphorylated Ser250. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2816474 |
Product Specific Information
This antibody detects endogenous levels of Smad2 only when phosphorylated at Serine 250.
Target Information
SMAD2 (MADH2, MAD2) regulates multiple cellular processes such as cell proliferation, apoptosis, and differentiation. It interacts with TGF-beta receptors through the SMAD anchor for receptor activation (SARA). Phosphorylation of SMAD2 by TGF-beta receptors induces dissociation from SARA and association with SMAD4, enabling nuclear translocation and transcriptional regulation. SMAD2 can also be phosphorylated by activin type 1 receptor kinase and mediates activin signaling. Alternatively spliced transcript variants encoding SMAD2 have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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