Thermo Fisher Scientific HFE Recombinant Rabbit Monoclonal Antibody (2O7D2)
HFE 단백질을 인식하는 토끼 유래 재조합 단클론 항체로, Western blot과 ELISA에 적합합니다. 인간 시료에서 반응하며, 고순도 친화 크로마토그래피로 정제되었습니다. 세포막 단백질 검출에 유용하며, 안정적인 PBS/glycerol buffer에 보관됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 2O7D2 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 249–348 of human HFE (Q30201) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2911778 |
Product Specific Information
- Positive test controls: HeLa, HT-29, A-549
- Target location: Cell membrane, Single-pass type I membrane protein
Immunogen sequence:
KEFEPKDVLP NGDGTYQGWI TLAVPPGEEQ RYTCQVEHPG LDQPLIVIWE PSPSGTLVIG VISGIAVFVV ILFIGILFII LRKRQGSRGA MGHYVLAERE
Target Information
The protein encoded by the HFE gene is a membrane protein similar to MHC class I-type proteins and associates with beta2-microglobulin. It regulates iron absorption by modulating the interaction of the transferrin receptor with transferrin. Mutations in this gene cause hereditary haemochromatosis, a recessive iron storage disorder. Multiple alternatively spliced variants have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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