Thermo Fisher Scientific H3K9me2 Polyclonal Antibody
H3K9me2에 특이적인 Rabbit Polyclonal 항체로, Western blot, IHC, ICC, ELISA, ChIP 등 다양한 응용에 적합합니다. Human, Mouse, Rat 반응성을 가지며, 액상 형태로 제공됩니다. Affinity Chromatography로 정제되어 높은 특이성과 재현성을 보장합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| ChIP assay (ChIP) | 5 µg antibody for 5–10 µg of Chromatin |
| ChIP-sequencing (ChIP-Seq) | 1:50–1:200 |
| Dot blot (DB) | 1:500–1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1–100 of human histone H3 (NP_003520.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.41 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914382 |
Product Specific Information
- Positive test controls: HeLa, NIH/3T3, C6
- Target location: Chromosome, Nucleus
- Immunogen sequence: ARTKQTARKS TGGKAPRK
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA to form the nucleosome structure. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translational modifications of histones can alter chromatin structure or enable binding of specific transcription factors. The N-terminal tail of histone H3 can undergo methylation, acetylation, and phosphorylation, influencing various cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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