Thermo Fisher Scientific CD14 Monoclonal Antibody (61D3), Brilliant Violet 480, eBioscience

Thermo Fisher Scientific CD14 Monoclonal Antibody (61D3), Brilliant Violet 480, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 615, 661, 737, 805
• Brilliant Violet 421, 605, 650, 711, 786
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue 510, 555, 585, 610-30S, 660-40S, 800
• NovaFluor Yellow 570, 660
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The 61D3 monoclonal antibody reacts with human CD14, a 53–55 kDa GPI-linked glycoprotein expressed on monocytes, interfollicular macrophages, and some dendritic cells. Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through surface CD14.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and tested for flow cytometry using human peripheral blood cells. Recommended use: 5 µL (0.5 µg) per 100 µL test volume with 10⁵–10⁸ cells/test.

Brilliant Violet™ 480 (BV480) emits at 479 nm and is intended for cytometers equipped with a 405 nm violet laser. Ensure instrument compatibility.

When using multiple polymer dye-conjugated antibodies (e.g., Super Bright™, Brilliant Violet™, Brilliant UV™), use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42)
• Brilliant Stain Buffer™ (Product # 00-4409-75)
  to minimize non-specific polymer interactions.

Note: BV480 is not compatible with methanol-based fixation.
Excitation: 440 nm; Emission: 479 nm; Laser: Violet.

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD14 is a 55 kDa GPI-anchored glycoprotein expressed on mature monocytes, macrophages, and neutrophils. It functions as a multifunctional lipopolysaccharide (LPS) receptor and exists both as membrane-bound and soluble forms. CD14 binds LPS via LBP, contributing to immune recognition and inflammatory responses. Soluble CD14 (sCD14) can distinguish structural variations in LPS and plays a role in neutralizing foreign LPS in serum. It also binds apoptotic cells and participates in allergic, inflammatory, and infectious processes. Alternative splicing yields multiple transcript variants encoding the same isoform. CD14 dysfunction is linked to infections and inflammatory diseases such as Mycobacterium chelonae infection and Croup.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지