Thermo Fisher Scientific Phospho-TRPM7 (Ser1513) Polyclonal Antibody
Phospho-TRPM7 (Ser1513) 특이적 폴리클로날 항체로, 인간·마우스·랫에서 반응하며 Western blot에 적합합니다. 항원 친화 크로마토그래피로 정제되었고, 인산화된 TRPM7 단백질 검출에 유용합니다. 연구용으로만 사용 가능합니다.
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Applications
- Western Blot (WB): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phospho-TRPM7 (Ser-1513) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Ser-1513 in human TRPM7. This site is well conserved in rat and mouse TRPM7, but has low homology to other TRPM family members. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2943007 |
Product Specific Information
The antibody was cross-adsorbed to unphosphorylated TRPM7 (Ser-1513) peptide and then affinity purified using phospho-TRPM7 (Ser-1513) peptide.
This antibody detects a 220 kDa protein on SDS-PAGE immunoblots of human MDA-MB-231 and rat PC12 cells stimulated with calyculin A, as well as a 70 kDa autophosphorylated recombinant kinase domain of human TRPM7.
These reactivities are not observed after lambda phosphatase treatment to dephosphorylate TRPM7.
Target Information
TRPCs are mammalian homologs of the Drosophila transient receptor potential (trp) protein and function as ion channels mediating capacitative calcium entry.
TRP-PLIK is both an ion channel and a kinase, conducting calcium and monovalent cations to depolarize cells and increase intracellular calcium.
Its kinase activity enables autophosphorylation and phosphorylation of other substrates, which is essential for channel function, as shown by its dependence on intracellular ATP and by kinase mutants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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