Thermo Fisher Scientific MMP14 Polyclonal Antibody
MMP14 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 사용 가능. Human 시료 검증 완료. 비결합형 액상 제품으로 4°C 단기 보관, -20°C 장기 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500 | - |
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:30 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | 1 µg/mL | - |
| Flow Cytometry (Flow) | Assay-dependent | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from internal region of human MMP-14 protein (UniProt ID: P50281-1, antigen range: 150–250). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2145181 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes. After antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature. Suggested positive controls include placenta or breast carcinoma.
Target Information
Matrix metalloproteinase (MMP) family proteins are involved in the breakdown of extracellular matrix during normal physiological processes such as embryonic development, reproduction, and tissue remodeling, as well as in pathological conditions including arthritis and metastasis. Most MMPs are secreted as inactive proproteins that are activated by extracellular proteinases. The MMP14 gene encodes a membrane-type MMP (MT-MMP) containing a transmembrane domain, indicating its expression at the cell surface. MMP14 activates MMP2, contributing to tumor invasion processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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