Thermo Fisher Scientific MMP15 Polyclonal Antibody
Rabbit polyclonal antibody targeting MMP15, reactive with human and mouse samples. Suitable for IHC and ICC applications. Antigen affinity purified and supplied in PBS with BSA and sodium azide. Recommended heat-mediated antigen retrieval for optimal s...
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide from the catalytic domain of mouse MMP-15 / MT2-MMP protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2145352 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes followed by cooling at room temperature for 20 minutes. After antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature. A suggested positive control is placenta tissue.
Target Information
Matrix metalloproteinases (MMPs) are enzymes involved in extracellular matrix degradation. Sixteen members are known, with ten existing in soluble form and MT-MMPs as integral membrane proteins. MT1-MMP, MT2-MMP, and MT3-MMP (also known as MMP14, MMP15, and MMP16, respectively) contain a C-terminal transmembrane domain anchoring them to the cell surface and an 8-amino-acid insert in their catalytic domain. MT1-MMP activates progelatinase A (MMP-2) and progelatinase B. MT2-MMP plays a minor role in activating pro-MMP-2 in breast carcinomas. Coexpression of MT1-MMP and MT2-MMP in advanced breast carcinomas suggests involvement of MT2-MMP in metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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