Thermo Fisher Scientific PDI Monoclonal Antibody (RL90)
PDI 단백질을 검출하는 RL90 클론의 Mouse Monoclonal Antibody. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합. 인간, 설치류, 돼지 등 여러 종에 반응. 단백질 A로 정제된 액상 형태로 -20°C 보관.
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- MA3019
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Thermo Fisher Scientific PDI Monoclonal Antibody (RL90)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:200–1:2,000 | 22 |
| Immunohistochemistry (IHC) | - | 12 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | 1 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100 | 1 |
| Immunocytochemistry (ICC/IF) | 1:100 | 42 |
| Flow Cytometry (Flow) | 0.5–1 µg/test | 1 |
| Immunoprecipitation (IP) | Assay-dependent | 3 |
| Neutralization (Neu) | - | 5 |
| Immunomicroscopy (IM) | Assay-dependent | - |
| Competition Assay (CA) | - | 1 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Hamster, Human, Mouse, Pig, Rat |
| Published Species | Dog, Hamster, Human, Mouse, Non-human primate, Rat, Rhesus monkey |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RL90 |
| Immunogen | Purified rat PDI protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2163120 |
Available Formats
- Alexa Fluor 488 (MA3-019-A488)
- Alexa Fluor 555 (MA3-019-A555)
- Alexa Fluor 647 (MA3-019-A647)
- Request custom conjugation
Product Specific Information
MA3-019 detects protein disulphide-isomerase (PDI) from human, rat, porcine, and mouse tissues as well as hamster cells.
It has been successfully used in Western blot, immunofluorescence, immunohistochemical, flow cytometry, and immunoprecipitation procedures.
By Western blot, this antibody detects a protein at 59 kDa representing PDI from rat liver extract or a slightly higher protein at 61 kDa representing PDI from human liver extract.
Immunohistochemical staining of PDI in rat intestine with MA3-019 yields a pattern consistent with cytoplasmic staining.
In immunoprecipitation procedures, MA3-019 has been shown to inhibit the activity of PDI in vitro.
MA3-019 also inhibits disulfide bond reduction of the HIV protein gp120 at the cell surface of CHO cells and human lymphoid cells.
In immunohistochemistry procedures, formalin-fixed paraffin-embedded tissue sections are recommended.
The MA3-019 antigen is purified rat PDI.
Target Information
Protein disulfide isomerase (PDI) is a multifunctional protein that catalyzes the formation, breakage, and rearrangement of disulfide bonds.
At the cell surface, it acts as a reductase that cleaves disulfide bonds of proteins attached to the cell, causing structural modifications of exofacial proteins.
Inside the cell, PDI forms or rearranges disulfide bonds of nascent proteins. At high concentrations, it functions as a chaperone that inhibits aggregation of misfolded proteins, while at low concentrations it facilitates aggregation (anti-chaperone activity).
PDI may be involved with other chaperones in structural modification of the TG precursor in hormone biogenesis.
It also acts as a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein (MTTP).
Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state, and enhancing cell migration.
For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.
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