Thermo Fisher Scientific PDI Monoclonal Antibody (RL90)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:2,000

View 22 publications 22 publications

Immunohistochemistry (IHC)

-

View 12 publications 12 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:100

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

View 42 publications 42 publications

Flow Cytometry (Flow)

0.5-1 µg/test

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 3 publications 3 publications

Neutralization (Neu)

-

View 5 publications 5 publications

Immunomicroscopy (IM)

Assay-dependent

-

Competition Assay (CA)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Hamster, Human, Mouse, Pig, Rat

Published species

Dog, Hamster, Human, Mouse, Non-human primate, Rat, Rhesus monkey

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

RL90

Immunogen

Purified rat PDI protein.

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor 488
• Alexa Fluor 555
• Alexa Fluor 647
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2163120

Product Specific Information

MA3-019 detects protein disulphide-isomerase (PDI) from human, rat, porcine and mouse tissues as well as hamster cells.

MA3-019 has been successfully used in Western blot, immunofluorescence, immunohistochemical, flow cytometry, and immunoprecipitation procedures. By Western blot, this antibody detects a protein at 59 kDa representing PDI from rat liver extract or a slightly higher protein at 61 kDa representing PDI from human liver extract. Immunohistochemical staining of PDI in rat intestine with MA3-019 yields a pattern consistent with cytoplasmic staining. In immunoprecipitation procedures MA3-019 has been shown to inhibit the activity of PDI in vitro. MA3-019 has also been found to inhibit disulfide bond reduction of the HIV protein, gp120, at the cell surface of CHO cells and human lymphoid cells.

In immunohistochemistry procedures, formalin-fixed paraffin-embedded tissue sections are recommended.

The MA3-019 antigen is purified rat PDI.

Target Information

Protein disulfide isomerase (PDI) is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, the protein seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. It may therefore cause structural modifications of exofacial proteins. Inside the cell, PDI seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, it functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity). PDI may be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. It also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.