Thermo Fisher Scientific HSP27 Monoclonal Antibody (G3.1)
HSP27 단백질을 검출하는 Thermo Fisher Scientific의 G3.1 단클론 항체입니다. Western blot, IHC, ELISA 등 다양한 응용에 사용 가능하며, 인간, 개, 설치류 등 여러 종에서 반응합니다. 고품질 액상 형태로 제공되며 연구용으로 최적화되어 있습니다.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific HSP27 Monoclonal Antibody (G3.1)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | 13 publications |
| Immunohistochemistry (IHC) | - | 13 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500 | - |
| Immunocytochemistry (ICC/IF) | 1:500 | 1 publication |
| ELISA | Assay-dependent | 2 publications |
| Immunoprecipitation (IP) | 2 µL | 3 publications |
| Immunomicroscopy (IM) | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Non-human primate, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | G3.1 |
| Immunogen | Partially purified human HSP27 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_325463 |
Product Specific Information
MA3-015 detects heat shock protein 27 kDa (HSP27) from human, rat, mouse, monkey, and canine samples.
It has been successfully used in Western blot, immunohistochemistry (paraffin), immunoprecipitation, immunofluorescence, immunocytochemistry, and ELISA procedures.
By Western blot, this antibody detects an ~24 kDa protein representing HSP27 in MCF-7 cell extract.
Immunohistochemical staining of human cervix with MA3-015 results in intense cytoplasmic staining of the epithelium within the parabasal cell layer.
Western blot detection of HSP27 in canine heart samples is weak and may require optimization.
The antigen is partially purified HSP27 derived from MCF-7 cytosol. The mouse HSP27 sequence is strongly conserved within the human sequence, so cross-reactivity with mouse HSP27 is expected.
Antibodies to this protein were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit.
This replacement antibody is recommended for researchers performing high content cell-based assays.
It has been validated for cellular immunofluorescence (IF) applications. Further optimization, including selection of the most appropriate fluorescent Dylight-conjugated secondary antibody, may be required for high content assays.
Target Information
In response to environmental stress, cells increase the expression of heat shock or stress proteins.
HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein functioning as a molecular chaperone that maintains denatured proteins in a folding-competent state.
It plays a role in stress resistance and actin organization, regulating processes such as phosphorylation and axonal transport of neurofilament proteins.
Mutations in the HSPB1 gene are associated with Charcot-Marie-Tooth disease 2F and distal hereditary motor neuronopathy 2B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.