Thermo Fisher Scientific HSP27 Monoclonal Antibody (G3.1)

Applications and Tested Dilution

Product Specifications

Product Specific Information

MA3-015 detects heat shock protein 27 kDa (HSP27) from human, rat, mouse, monkey, and canine samples.
It has been successfully used in Western blot, immunohistochemistry (paraffin), immunoprecipitation, immunofluorescence, immunocytochemistry, and ELISA procedures.
By Western blot, this antibody detects an ~24 kDa protein representing HSP27 in MCF-7 cell extract.
Immunohistochemical staining of human cervix with MA3-015 results in intense cytoplasmic staining of the epithelium within the parabasal cell layer.
Western blot detection of HSP27 in canine heart samples is weak and may require optimization.

The antigen is partially purified HSP27 derived from MCF-7 cytosol. The mouse HSP27 sequence is strongly conserved within the human sequence, so cross-reactivity with mouse HSP27 is expected.

Antibodies to this protein were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit.
This replacement antibody is recommended for researchers performing high content cell-based assays.
It has been validated for cellular immunofluorescence (IF) applications. Further optimization, including selection of the most appropriate fluorescent Dylight-conjugated secondary antibody, may be required for high content assays.

Target Information

In response to environmental stress, cells increase the expression of heat shock or stress proteins.
HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein functioning as a molecular chaperone that maintains denatured proteins in a folding-competent state.
It plays a role in stress resistance and actin organization, regulating processes such as phosphorylation and axonal transport of neurofilament proteins.
Mutations in the HSPB1 gene are associated with Charcot-Marie-Tooth disease 2F and distal hereditary motor neuronopathy 2B.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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