Thermo Fisher Scientific Ly-49H Monoclonal Antibody (3D10), PE, eBioscience
마우스 NK 세포의 활성 수용체 Ly49H를 인식하는 단클론 항체로, PE 형광표지되어 유세포분석(Flow Cytometry)에 적합합니다. C57BL/6 마우스에서 검증되었으며, 비특이적 반응이 적고 고순도 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 0.25 µg/test | View 4 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3D10 |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation/Emission Max | 565/576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2572636 |
Additional Formats
Product Specific Information
The monoclonal antibody 3D10 recognizes Ly49H, a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer (NK) cells. Ly49H, like Ly49D, lacks the ITIM motif and contains an arginine in the transmembrane domain, suggesting it functions as an activation receptor.
- Expressed in a subset of NK cells but not NKT (NK1.1⁺/CD3⁺) cells
- Expression confirmed in C57BL/6 and NWNA mice, not in Balb/c or DBA/2 strains
- No cross-reactivity with Ly49A, C, D, or G2
- Addition of 3D10 antibody to cell cultures induces lysis of target cells
Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometry using C57BL/6 splenocytes. Recommended ≤1.0 µg/test in 100 µL volume. Optimize titration as needed.
Each NovaFluor conjugate or kit is shipped with CellBlox Blocking Buffer.
Use 5 µL per sample for optimal staining, including single-color controls.
Excitation: 509 nm
Emission: 665 nm
Laser: 488 nm (Blue)
NovaFluor conjugates utilize Phiton™ technology, designed for engineered fluorescent signatures minimizing spillover and spread.
Learn more about NovaFluor dyes
Target Information
Ly49H is a C-type lectin receptor expressed on NK cells. It acts as an activation receptor and is not expressed on NKT (NK1.1⁺/CD3⁺) cells. Natural killer cells can express multiple Ly49 family proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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