Thermo Fisher Scientific Dendritic Cell Marker DCIR2 Monoclonal Antibody (33D1), PE, eBioscience

Applications

Immunohistochemistry (Frozen) (IHC (F))

• Tested Dilution: N/A
• Publications: View 1 publication

Flow Cytometry (Flow)

• Tested Dilution: 0.25 µg/test
• Publications: View 6 publications

Product Specifications

Additional Formats

• Unconjugated
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The 33D1 monoclonal antibody reacts with a mouse dendritic cell (DC)-specific surface marker, DCIR2. The nature and biological activity of DCIR2 is unknown. DCIR2 has been reported on various dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer’s patch. Bone marrow dendritic cells require GM-CSF to express DCIR2, and this expression is downregulated in the presence of IL-4. DCIR2 has also been detected in vivo in brain dendritic cells post infection with Toxoplasma gondii.

Applications Reported: The 33D1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes at ≤0.25 µg/test (in 100 µL final volume). Cell number should be determined empirically (10⁵–10⁸ cells/test). Antibody titration is recommended for optimal performance.

Fluorescence Characteristics:

• Excitation: 488–561 nm
• Emission: 578 nm
• Compatible Lasers: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

The biological activity of DCIR2 remains unknown. It is expressed on dendritic cell subpopulations in mouse thymus, spleen, lymph node, and Peyer’s patch. Expression in bone marrow DCs requires GM-CSF and is downregulated by IL-4. It has also been detected in brain DCs following Toxoplasma gondii infection.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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