Thermo Fisher Scientific CD314 (NKG2D) Monoclonal Antibody (CX5), PE, eBioscience

Thermo Fisher Scientific CD314 (NKG2D) Monoclonal Antibody (CX5), PE, eBioscience

Applications

• Reported Use: Flow Cytometric Analysis
• Tested Application: Flow Cytometry (Flow)
• Tested Dilution: ≤ 0.25 µg/test
• Publications: View 33 publications

Product Specifications

Available Formats

• Alexa Fluor 488
• APC
• Functional Grade
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

Description:
CX5 monoclonal antibody recognizes mouse NKG2D, a lectin-like molecule expressed on NK cells. Mouse NKG2D binds to RAE-1 (α, β, γ, δ, ε) and H60 molecules, and can costimulate multiple NK activation receptors through DAP12/DAP10 adaptor molecules. NKG2D is expressed by spleen and liver NK cells, NK1.1⁺ thymocytes, activated LAK cells, and a subset of splenic NKT cells.

Applications Reported:
CX5 antibody is reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤ 0.25 µg per test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test. Antibody titration is recommended for optimal performance.

Excitation/Emission:
488–561 nm / 578 nm (Blue, Green, Yellow-Green Laser)

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

Natural killer (NK) cells mediate lysis of tumor and virus-infected cells without prior activation. NK cells regulate humoral and cell-mediated immunity and express calcium-dependent (C-type) lectins involved in NK function. The NK gene encodes a member of the NKG2 family, a type II transmembrane protein with a C-type lectin domain. The NKG2 gene family resides within the NK complex region containing several NK-specific lectin genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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