Thermo Fisher Scientific CD3 Monoclonal Antibody (eBioG4.18 (G4.18)), APC, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.25 µg/test
  View 10 publications

Product Specifications

Additional Formats

• Unconjugated
• Biotin
• FITC
• Functional Grade
• PE
• PerCP-eFluor 710
• Custom conjugation available

Product Specific Information

The eBioG4.18 monoclonal antibody recognizes the rat CD3 protein, a critical component of the T-cell receptor (TCR) and a marker of the T-cell lineage. CD3 participates in signal transduction from the TCR to the nucleus, inducing responses such as IL-2 transcription and clonal expansion.

• In vitro: Immobilized G4.18 antibody activates T cells; soluble form inhibits allogeneic mixed-lymphocyte proliferation and cytotoxicity.
• In vivo: Demonstrated to induce long-term specific tolerance to organ allografts.
• Applications Reported: Suitable for flow cytometric analysis.
• Applications Tested: Tested with rat splenocytes at ≤0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
  Recommended careful titration for optimal performance.
• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

The CD3 subunit complex is essential for transducing antigen-recognition signals into T cells and regulating TCR surface expression. It includes CD3 gamma, delta, epsilon, and zeta subunits—members of the immunoglobulin superfamily encoded on human chromosome 11.

• CD3 subunits have long cytoplasmic tails associated with signal transduction molecules via double tyrosine-based motifs.
• CD3 plays roles in TCR-induced growth arrest, cell survival, and proliferation.
• Expressed on 68–82% of peripheral blood lymphocytes and 65–85% of thymocytes; absent on B and NK cells.
• Decreased T lymphocyte percentages may occur in autoimmune diseases.
• Defects in CD3 genes are linked to CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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