Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (J43), PE, eBioscience
마우스 PD-1 단백질을 인식하는 J43 모노클론 항체로, PE 형광 표지되어 있습니다. Flow Cytometry에 최적화되어 있으며, 0.5 µg/test로 사용 가능합니다. 면역 조절 연구 및 자가면역 질환 모델 분석에 유용합니다. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (J43), PE, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 78 publications |
| Functional Assay (Functional) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | J43 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466294 |
Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 563 / 615 / 661 / 737 / 805
- Brilliant Violet 480 / 605 / 650 / 786
- eFluor 450 / 506
- FITC
- Functional Grade
- PE-Cyanine7 / PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
- Custom conjugation available
Product Specific Information
The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes.
Two novel B7 family members, PD-L1 (B7-H1) and PD-L2 (B7-DC), serve as PD-1 ligands. J43 inhibits the binding of mouse PD-L1-Ig and PD-L2-Ig to PD-1/BHK transfected cells and can modulate immune responses in vivo, including exacerbation of GVHD, EAE, and NOD diabetes models.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on stimulated mouse splenocytes at ≤0.5 µg/test (100 µL final volume).
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
PD-1 (PDCD-1) is a receptor expressed on activated T cells that mediates inhibitory signals upon binding to its ligands PD-L1 and PD-L2 on antigen-presenting cells. This interaction downregulates immune activation in the absence of danger signals. PD-1 deficiency leads to hyperactivated immune responses and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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