Thermo Fisher Scientific HLA-ABC Monoclonal Antibody (W6/32), PE, eBioscience
인간 MHC class I (HLA-A, B, C)를 인식하는 W6/32 단클론 항체로, PE 형광 표지되어 있습니다. Flow cytometry에 최적화되어 있으며, 5 µL/test로 사용 가능합니다. 모든 핵세포에서 발현되는 HLA class I 항원을 검출하는 데 유용합니다.
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Applications
Immunohistochemistry (Frozen) (IHC (F))
- Tested Dilution: Not specified
- Publications: View 2 publications
Flow Cytometry (Flow)
- Tested Dilution: 5 µL (0.25 µg)/test
- Publications: View 41 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse, Rhesus monkey |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | W6/32 |
| Conjugate | PE |
| Excitation/Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10545857 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- Biotin
- Brilliant UV 395 / 615 / 737
- Brilliant Violet 650 / 786
- FITC
- Functional Grade
- NovaFluor (Blue, Red, Yellow series)
- PE-Cyanine5 / PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436 / 600 / 702
- Custom conjugation available
Product Specific Information
The W6/32 monoclonal antibody reacts with human major histocompatibility complex (MHC) class I antigens (HLA-A, -B, -C). These antigens, associated with beta 2-microglobulin, are expressed by all human nucleated cells and are central to cell-mediated immune response and tumor surveillance.
W6/32 recognizes a non-polymorphic epitope shared among HLA-A, -B, and -C loci and immunoprecipitates both 43 kDa and 11–12 kDa chains. Cross-reactivity is observed in baboon, rhesus, and cynomolgus monkey.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for use in flow cytometry (5 µL per test).
A test is defined as the amount of antibody that stains a cell sample in a final volume of 100 µL. Typical cell numbers range from 10⁵ to 10⁸ cells/test.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells, noncovalently associated with beta2-microglobulin. They present foreign peptides for recognition by cytotoxic T cells, playing key roles in antiviral and antitumor defense.
HLA-class I molecules include HLA-A, HLA-B, and HLA-C.
HLA and MHC antibodies are critical in Immunopeptidomics, aiding in the identification and characterization of neoantigens via LC-MS/MS.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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