
Thermo Fisher Scientific PDI Recombinant Rabbit Monoclonal Antibody (JF97-08)
PDI 단백질을 인식하는 재조합 토끼 단클론 항체(JF97-08)로, Western blot 및 유세포분석에 적합합니다. 높은 특이성과 재현성을 제공하며, lot 간 일관성이 우수합니다. Protein A 정제, 액상 형태, 1 mg/mL 농도로 제공됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JF97-08 |
| Immunogen | Synthetic peptide within Human PD1 (aa 405–450) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809819 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning antibody DNA sequences from immunoreactive rabbits. Selected clones are screened for optimal performance.
Key advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Protein disulfide isomerase (PDI) is a multifunctional enzyme that catalyzes the formation, breakage, and rearrangement of disulfide bonds.
- At the cell surface, PDI acts as a reductase, modifying exofacial proteins.
- Inside the cell, it assists in disulfide bond formation and rearrangement in nascent proteins.
- At high concentrations, it functions as a chaperone preventing aggregation of misfolded proteins, while at low concentrations it may promote aggregation.
- PDI also participates in hormone biogenesis and serves as a structural subunit in enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein (MTTP).
- It interacts with LGALS9, retaining P4HB at the cell surface of Th2 T helper cells, enhancing disulfide reductase activity and cell migration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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