Thermo Fisher Scientific beta Actin Recombinant Rabbit Monoclonal Antibody (JF53-10), HRP
Western blot용 HRP 결합 베타 액틴 항체로, 사람, 생쥐, 랫드 등 다양한 종에 반응합니다. 재조합 토끼 단클론 항체로 높은 특이성과 재현성을 제공합니다. 단백질 로딩 컨트롤 및 세포골격 연구에 적합합니다.
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Applications
- Western Blot (WB)
Tested dilution: 1:20,000
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Hamster, Human, Mouse, Non-human primate, Plant, Rat, Zebrafish |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JF53-10 |
| Immunogen | Recombinant full-length protein of Human beta Actin (aa 1–375) |
| Conjugate | HRP |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809817 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the large rabbit immune repertoire
Target Information
Beta actin is one of six actin isoforms identified in humans. It is a non-muscle cytoskeletal protein expressed in all human cell types and plays a key role in cell motility, structure, and integrity.
Beta and gamma actins coexist in most cell types as cytoskeletal components. Polymerization of globular actin (G-actin) forms filamentous actin (F-actin), a two-stranded helical structure.
Because beta actin is ubiquitously expressed, it is frequently used as a loading control in protein detection assays such as Western blotting.
Antibodies to beta actin are valuable tools for studying intracellular distribution and the dynamic aspects of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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