Thermo Fisher Scientific GAPDH Recombinant Rabbit Monoclonal Antibody (JF81-04), HRP
HRP 결합형 GAPDH 재조합 토끼 단클론 항체로 Western blot에서 높은 민감도와 특이성을 제공합니다. HEK293 세포에서 발현되며, 로트 간 일관성과 동물 유래 성분이 없는 제형입니다. 단백질 로딩 컨트롤용으로 적합합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:50,000
- View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chicken, Fish, Human, Mouse, Non-human primate, Rat |
| Published Species | Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JF81-04 |
| Immunogen | Recombinant full length protein of Human GAPDH (aa 1–335) |
| Conjugate | HRP |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809816 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme involved in glycolysis, existing as a tetramer of 37-kDa subunits. It catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphate in the presence of NADPH.
Beyond glycolysis, GAPDH participates in:
- DNA replication and repair
- Nuclear RNA export
- Membrane fusion and microtubule bundling
- Gene expression regulation during apoptosis
- Cellular processes in neurodegenerative diseases and cancer
GAPDH interacts with various proteins, including amyloid precursor protein (related to Alzheimer’s disease) and Huntingtin (Huntington’s disease). Due to its high expression in most tissues, GAPDH serves as a reliable protein loading control in Western blot analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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