Thermo Fisher Scientific iASSP Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000-1:5,000

-

ELISA (ELISA)

1:40,000-1:160,000

-

Immunoprecipitation (IP)

1:1,000-1:5,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 775-800 of human iASPP (isoform 1) protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PPP1R13L,uniProtId:Q8WUF5-1,ncbiNodeId:9606,antigenRange:775-800,antigenLength:828,antigenImageFileName:600-401-926_PPP1R13L_Q8WUF5-1_Rabbit.svg,antigenImageFileNamePDP:600-401-926_PPP1R13L_Q8WUF5-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.1 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity-purified antibody is directed against the human iASPP protein. A BLAST analysis was used to suggest cross reactivity with iASPP proteins from human, dog and mouse sources. Expect reactivity against both isoform 1 and isoform 2. Partial reactivity may occur against iASPP from bovine. Reactivity against homologues from other sources is not known.

Target Information

IASPP is one of the most evolutionarily conserved inhibitors of p53 (TP53), whereas ASPP1. and ASPP2. are activators of p53.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.