Thermo Fisher Scientific Mdm2 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Store vial at -20°C prior to opening.
Aliquot contents and freeze at -20°C or below for extended storage.
Avoid cycles of freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature.
Stable for several weeks at 4°C as an undiluted liquid.
Dilute only prior to immediate use.

This affinity-purified antibody is directed against mouse MDM2 protein. Reactivity occurs against Mouse MDM2 protein. BLAST analysis suggests minimal cross-reactivity with MDM2 homologues from other species.

Target Information

MDM2 is a ubiquitin ligase for p53 and plays a central role in regulating p53 stability. It is located on chromosome 12q. Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 enhances its interaction with p300, facilitating MDM2-mediated ubiquitination and degradation of p53.
Phosphorylation also blocks MDM2 binding to p19ARF, increasing p53 degradation. MDM2 negatively regulates p53 function and overexpression can inactivate p53, reducing its tumor suppressor activity.
MDM2 participates in apoptosis and cell cycle regulation and is overexpressed in various human malignancies, including soft tissue carcinomas and breast cancer. It also interacts with proteins such as retinoblastoma 1 and ribosomal protein L5. Over 40 alternatively spliced transcript variants of MDM2 have been identified in tumor and normal tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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