Thermo Fisher Scientific Phospho-EGFR (Tyr1197) Polyclonal Antibody
EGFR 단백질의 Tyr1197 인산화 형태를 특이적으로 인식하는 폴리클로날 항체입니다. Western blot, IHC, ELISA 등 다양한 응용에 적합하며, 인간 EGFR에 반응합니다. Affinity chromatography로 정제된 액상 항체로 -20°C에서 안정적으로 보관 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:250–1:1,500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL |
| ELISA | 1:4,000–1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Affinity purified antibody prepared from whole rabbit serum immunized with a synthetic peptide corresponding to the C-Terminus near amino acids 1175–1200 of human EGFR protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.55 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody is directed against the phosphorylated form of human EGFR protein at the pY1197 residue. Reactivity occurs against human EGFR pY1197 protein and the antibody is specific for the phosphorylated form. Reactivity with non-phosphorylated EGFR is minimal by ELISA.
BLAST analysis suggests cross-reactivity with phosphorylated EGFR from human, mouse, and rat sources. Reactivity with EGFR from other species is unknown.
Target Information
EGFR (epidermal growth factor receptor) is a receptor tyrosine kinase that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer, and EGFR is the target of both biological and small-molecule therapeutics.
EGFR is encoded by the EGFR gene located on chromosome 7 in humans and belongs to the HER/ERbB family, which includes ERbB2, ERbB3, and ERbB4.
Binding of ligands such as EGF and TGF-α to the extracellular domain leads to EGFR dimerization and autophosphorylation of tyrosine residues in the cytoplasmic domain.
Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, and squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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