Thermo Fisher Scientific CD45R (B220) Monoclonal Antibody (RA3-6B2), eFluor 506, eBioscience

Thermo Fisher Scientific CD45R (B220) Monoclonal Antibody (RA3-6B2), eFluor 506, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV (395–805), Brilliant Violet (421–786)
• eFluor 450, 570, 615, 660
• FITC
• Functional Grade
• NovaFluor Series (Blue, Red, UV, Violet, Yellow)
• PE, PE-Cyanine Series, PE-eFluor 610
• PerCP Series
• Super Bright Series
• Custom conjugation available

Product Specific Information

The RA3-6B2 monoclonal antibody reacts with the exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule.
CD45R/B220 is primarily expressed by B cell lineage from early Pro-B to mature B cells, and also by some activated T cells, LAK cells, NK progenitors, and certain mutant T cells.

• Reported application: Flow cytometric analysis
• Tested on mouse splenocytes (≤0.5 µg/test, 100 µL final volume)
• Recommended titration for optimal performance
• eFluor® 506 excitation: 405 nm; emission: 506 nm; violet laser compatible
• Suggested detection filter: 510/20 band pass
• Post-manufacturing filtration: 0.2 µm

Target Information

CD45R (PTPRC) is a protein tyrosine phosphatase regulating cell growth, differentiation, and immune signaling.
It is expressed in hematopoietic cells and critical for T- and B-cell receptor signaling.
CD45 isoforms vary by alternative splicing and cell type, influencing membrane localization and signaling.
CD45 also modulates integrin-mediated pathways and apoptosis-related DNA fragmentation.
Diseases linked to CD45 dysfunction include Severe Combined Immunodeficiency and Hepatitis C.

For Research Use Only. Not for use in diagnostic procedures.

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