Thermo Fisher Scientific CD44 Monoclonal Antibody (IM7), eFluor 506, eBioscience
CD44 단백질을 인식하는 쥐 단클론 항체(IM7)로, eFluor 506 형광 표지되어 있습니다. Flow cytometry에 최적화되어 있으며, 405 nm 레이저로 여기됩니다. 다양한 세포 유형의 CD44 발현 분석에 활용 가능합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 11 publications |
| Immunocytochemistry (ICC/IF) | - | View 9 publications |
| Flow Cytometry (Flow) | 1 µg/test | View 107 publications |
| Neutralization (Neu) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Not Applicable |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), eFluor™ 506, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | IM7 |
| Conjugate | eFluor™ 506 |
| Excitation / Emission Max | 419 / 508 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_2762675 |
Available Formats
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV / Violet / Blue / Red / Yellow series
- FITC, PE, PE-Cyanine5, PE-Cyanine7
- PerCP-Cyanine5.5
- Super Bright series
- Functional Grade
- Request custom conjugation
Product Specific Information
The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen, and peripheral B and T cells can upregulate CD44 expression. CD44 functions as an adhesion molecule through binding to hyaluronate, an extracellular matrix component.
Applications Reported: This IM7 antibody has been reported for use in flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes. Use ≤1 µg per test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test. Optimize titration for best performance.
Fluorophore Information:
eFluor™ 506 is excited with the violet laser (405 nm) and emits at 506 nm. Recommended filter: 510/20 band pass or equivalent. Ensure instrument compatibility.
Excitation: 405 nm
Emission: 506 nm
Laser: Violet
Target Information
CD44 is a 100 kDa type I transmembrane glycoprotein expressed on human leukocytes, white matter of the brain, and some epithelial cells. It serves as a receptor for hyaluronic acid (HA) and mediates cell-cell interactions, adhesion, and migration. CD44 participates in lymphocyte activation, recirculation, and homing, and can be upregulated in certain carcinomas, potentially linked to metastasis.
CD44 expression occurs in hematopoietic and non-hematopoietic cells, epithelial tissues, and filopodia of cultured keratinocytes. Bone marrow myeloid cells and memory T cells express high levels of CD44; peripheral B and T cells can upregulate expression upon stimulation. Alternative splicing generates multiple isoforms with distinct functions.
Associated diseases: Superficial keratitis, lichen sclerosus, and possibly tumor metastasis formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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