Thermo Fisher Scientific CD45.1 Monoclonal Antibody (A20), eFluor 506, eBioscience
마우스 CD45.1 항원을 인식하는 A20 클론 단일클론 항체로, eFluor 506 형광체가 결합되어 있습니다. 유세포 분석용으로 검증되었으며, 405 nm 레이저에서 여기되어 506 nm에서 방출됩니다. 모든 백혈구에서 발현되는 CD45.1 검출에 적합합니다.
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Thermo Fisher Scientific CD45.1 Monoclonal Antibody (A20), eFluor 506, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 1 µg/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), eFluor™ 506 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A20 |
| Conjugate | eFluor™ 506 |
| Excitation / Emission Max | 419 / 508 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2637183 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395, 563, 661, 737, 805
- Brilliant Violet 421, 480, 605, 650, 711, 786
- eFluor 450
- FITC
- NovaFluor Blue 510, 610-30S, 660-40S
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436, 600, 645, 702
- Request custom conjugation available
Product Specific Information
The A20 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.1-expressing mouse strains such as SJL/J, DA, STS/A, and RIII. CD45.1 is expressed by all leukocytes in these strains.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes at ≤1 µg per test (100 µL final volume). Optimal titration recommended for best performance.
eFluor® 506 can be excited with the violet laser line (405 nm) and emits at 506 nm. Recommended band pass filter: 510/20 or equivalent. Ensure instrument compatibility.
- Excitation: 405 nm
- Emission: 506 nm
- Laser: Violet
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising ~10% of lymphocyte surface proteins. It regulates Src-family kinases, lymphocyte development, and antigen signaling. CD45 exists as multiple isoforms due to alternative splicing, differing in extracellular domains. It modulates integrin-mediated signaling, promotes cell survival, and participates in apoptosis-related DNA fragmentation. CD45RA is one such isoform with restricted expression among lymphoid subtypes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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