Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (J121), eBioscience
Human 및 Mouse에서 CD279 (PD-1) 검출용 단클론 항체. Western blot, IHC, ICC/IF에 사용 가능. 면역관문 연구 및 종양면역학 응용에 적합. Unconjugated 형태, 고순도(>90%)로 제공.
- 카탈로그번호
- 14-2798-82
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (J121), eBioscience
Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (J121)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | J121 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2572870 |
| Purity | >90%, determined by SDS-PAGE |
| Aggregation | <10%, determined by HPLC |
| Filtration | 0.2 µm post-manufacturing filtered |
Product Specific Information
This J121 monoclonal antibody reacts with human CD279 (programmed death-1, PD-1), a 55 kDa member of the CD28 immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T and B lymphocytes.
Two novel members of the B7 family have been identified as CD279 ligands: CD274 (PD-L1, B7-H1) and CD273 (PD-L2, B7-DC). These ligands exhibit overlapping functions and are constitutively expressed on some normal tissues and upregulated on activated antigen-presenting cells.
Therapies targeting the CD279/CD274 pathway have become a major focus for treatment of melanoma, renal cell carcinoma, Hodgkin’s lymphoma, and non-small cell lung carcinoma (NSCLC).
Note: The J121 monoclonal antibody is not recommended for flow cytometry of human cells. For flow cytometry detection of human CD279, please refer to clone eBioJ105 (J105).
Applications Reported: Suitable for western blotting and immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections.
Applications Tested: Tested for immunohistochemistry using high or low pH antigen retrieval; recommended ≤5 µg/mL. Titrate for optimal assay performance.
Target Information
Cell-mediated immune responses are initiated by T lymphocytes stimulated by peptides bound to MHC molecules on antigen-presenting cells (APC). Activated T cells express PD-1 (PDCD-1), which mediates inhibitory signals from APCs. PD-1 binds two ligands, PD-L1 and PD-L2, to inhibit immune activation in the absence of danger signals such as LPS. PD-1 deficiency leads to hyperactivated immune responses and autoimmune disease.
Despite its predicted molecular weight, PD-1 often migrates at a higher molecular weight in SDS-PAGE.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.