Thermo Fisher Scientific beta Catenin Monoclonal Antibody (15B8), eBioscience
Human 및 Mouse beta-catenin을 검출하는 15B8 단클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry 등에 사용 가능. Wnt 신호전달 및 세포접착 관련 연구에 적합하며, 고순도(>90%)와 안정적인 액상 형태로 제공.
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Thermo Fisher Scientific beta Catenin Monoclonal Antibody (15B8), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–5 µg/mL | 5 publications |
| Immunohistochemistry (IHC) | – | 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1 µg/mL | – |
| Immunocytochemistry (ICC/IF) | – | 3 publications |
| Flow Cytometry (Flow) | Assay-Dependent | 3 publications |
| Immunoprecipitation (IP) | Assay-Dependent | 1 publication |
| Miscellaneous PubMed (Misc) | – | 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 15B8 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865043 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Available Conjugated Formats
- Alexa Fluor 488
- eFluor 660
- PE
- Custom conjugation available upon request
Product Specific Information
The 15B8 monoclonal antibody reacts with human and mouse beta-catenin, a member of the catenin family involved in cellular adhesion and Wnt signaling. Beta-catenin binds to the cytoplasmic tail of E-cadherin and plays a crucial role in mediating cellular adhesion and gene regulation. Upon Wnt activation, beta-catenin translocates to the nucleus and interacts with transcription factors TCF and LEF to modulate gene expression.
Expression of beta-catenin occurs in both immune and non-immune tissues, including thymocytes and lymphocytes. The Wnt/beta-catenin pathway is essential for T, B, and hematopoietic stem cell development.
Applications Reported: Suitable for intracellular staining and flow cytometric analysis, immunoprecipitation, western blotting, and immunohistochemical staining of formalin-fixed paraffin-embedded tissues.
Applications Tested: Validated for western blot (Jurkat cell lysates, 1–5 µg/mL) and IHC (≤5 µg/mL, low or high pH antigen retrieval). Optimal titration recommended.
Target Information
Beta-catenin is an adherens junction protein critical for epithelial cell layer formation and intercellular adhesion. It interacts with E-cadherin and alpha-catenin, linking the adhesion complex to the actin cytoskeleton. Beta-catenin also associates with APC and microtubules, participating in multiple signaling pathways, including Wnt/Wingless.
Pathological roles of beta-catenin have been identified in pilomatrixoma, medulloblastoma, colorectal, ovarian, and other solid tumors. In the nucleus, beta-catenin co-activates Lef/Tcf transcription factors, promoting transcription of genes such as cyclin D and c-myc, which drive cell proliferation and tumorigenesis.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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