Thermo Fisher Scientific CD277 Monoclonal Antibody (eBioBT3.1 (20.1, BT3.1)), eBioscience

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 3 publications 3 publications

Immunoprecipitation (IP)

Assay-Dependent

-

Functional Assay (Functional)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

eBioBT3.1 (20.1, BT3.1)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865045

Product Specific Information

Description: The eBioBT3.1 monoclonal antibody recognizes BT3.1, BT3.2 and BT3.3. The BT family of proteins is a subgroup of the Ig superfamily (IgSF). BT is a glycoprotein that forms a major component of the milk fat globule molecule. In addition to BT, there are six other genes that have been discovered which have been sub-divided into the BT2 (BT2.1, BT2.2, and BT2.3) and BT3 (BT3.1, BT3.2, and BT3.3) families. BT3.1, BT3.2, and BT3.3 share 95% mRNA identity. BT3 molecules are constitutively expressed on the cell surface of antigen-presenting cells, and the IgV-like domain of BT3.1 is sufficient for interaction with its counter-receptor. BT3.1 is expressed by T cells, B cells and CD14+ cells.

Applications Reported: This eBioBT3.1 (20.1, BT3.1) antibody has been reported for use in flow cytometric analysis, and immunoprecipitation.

Applications Tested: This eBioBT3.1 (20.1, BT3.1) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The butyrophilin (BTN) genes are a group of major histocompatibility complex (MHC)-associated genes that encode type I membrane proteins with 2 extracellular immunoglobulin (Ig) domains and an intracellular B30.2 (PRYSPRY) domain. Three subfamilies of human BTN genes are located in the MHC class I region: the single-copy BTN1A1 gene and the BTN2 and BTN3 genes, which have undergone tandem duplication, resulting in 3 copies of each.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.