Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (eBioJ105 (J105)), eBioscience

Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (eBioJ105 (J105)), eBioscience

Applications and Tested Dilutions

Product Specifications

Available Formats

• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 615 / 661 / 737 / 805
• Brilliant Violet 421 / 480 / 650 / 711 / 786
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Custom conjugation available upon request

Product Specific Information

The J105 monoclonal antibody reacts with human PD-1 (programmed death-1), a 55 kDa member of the CD28 immunoglobulin superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. PD-1 is expressed predominantly on activated T and B lymphocytes. Two ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC), have overlapping functions and are expressed constitutively on some tissues and upregulated on activated antigen-presenting cells.

Costaining experiments suggest that eBioJ105 recognizes a different epitope than MIH4 (Product #11-9969).

Applications Reported:
Flow cytometry, immunoprecipitation, and immunohistology staining of frozen tissue sections.

Applications Tested:
Tested by flow cytometric analysis of PHA-stimulated normal human peripheral blood cells. Recommended ≤1 µg/test in 100 µL volume. Cell number may range from 10⁵–10⁸ cells/test. Titrate for optimal performance.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

T-cell activation is regulated by PD-1 (PDCD-1), which mediates inhibitory signals upon binding to its ligands PD-L1 and PD-L2 on antigen-presenting cells. These interactions suppress immune activation in the absence of danger signals. PD-1 knockout mice exhibit hyperactivated immune systems and autoimmune diseases. PD-1 often migrates at higher molecular weight in SDS-PAGE than predicted.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.