Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (eBioJ105 (J105)), eBioscience
휴먼 PD-1(CD279)을 인식하는 eBioJ105(J105) 모노클로날 항체로, Flow Cytometry, IHC, IP 등 다양한 응용에 적합. 비결합형 형태로 높은 순도(>90%) 및 낮은 응집도(<10%)를 보유. 면역조절 연구 및 T세포 활성 분석에 활용 가능.
- 카탈로그번호
- 14-2799-xx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (eBioJ105 (J105)), eBioscience
Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (eBioJ105 (J105)), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | 2 publications |
| Immunocytochemistry (ICC/IF) | - | 1 publication |
| Flow Cytometry (Flow) | 1 µg/test | 32 publications |
| ELISA | - | 1 publication |
| Immunoprecipitation (IP) | Assay-Dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rhesus monkey |
| Published Species | Baboon, Human, Mouse, Non-human primate, Rhesus monkey |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioJ105 (J105) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865049 |
Available Formats
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 615 / 661 / 737 / 805
- Brilliant Violet 421 / 480 / 650 / 711 / 786
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
- Custom conjugation available upon request
Product Specific Information
The J105 monoclonal antibody reacts with human PD-1 (programmed death-1), a 55 kDa member of the CD28 immunoglobulin superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. PD-1 is expressed predominantly on activated T and B lymphocytes. Two ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC), have overlapping functions and are expressed constitutively on some tissues and upregulated on activated antigen-presenting cells.
Costaining experiments suggest that eBioJ105 recognizes a different epitope than MIH4 (Product #11-9969).
Applications Reported:
Flow cytometry, immunoprecipitation, and immunohistology staining of frozen tissue sections.
Applications Tested:
Tested by flow cytometric analysis of PHA-stimulated normal human peripheral blood cells. Recommended ≤1 µg/test in 100 µL volume. Cell number may range from 10⁵–10⁸ cells/test. Titrate for optimal performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
T-cell activation is regulated by PD-1 (PDCD-1), which mediates inhibitory signals upon binding to its ligands PD-L1 and PD-L2 on antigen-presenting cells. These interactions suppress immune activation in the absence of danger signals. PD-1 knockout mice exhibit hyperactivated immune systems and autoimmune diseases. PD-1 often migrates at higher molecular weight in SDS-PAGE than predicted.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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