Thermo Fisher Scientific Cdc34 Polyclonal Antibody
Rabbit polyclonal antibody recognizing human CDC34 protein. Validated for WB, IHC(P), and ICC/IF. Supplied as liquid, 0.1 mg/mL in PBS with 40% glycerol. For research use only, not for diagnostic applications.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human CDC34. Recombinant protein control fragment (Product #RP-101860). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2789177 |
Product Specific Information
Immunogen sequence:
LNEPNTFSPA NVDASVMYRK WKESKGKDRE YTDIIRKQVL GTKVDAERDG VKVPTTLAEY CVKTKAPAPD EGSDLFYDDY YEDGEVEEEA DSCFGDDEDD SGT
Target Information
CDC34 accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. It catalyzes Lys-48-linked polyubiquitination and cooperates with E2 UBCH5C and the SCF (FBXW11) E3 ligase complex for polyubiquitination of NFKBIA, leading to its proteasomal degradation.
CDC34 participates in ubiquitin chain elongation, working with UBE2D3 as the initiator E2. It also cooperates with the SCF (SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1.
Additionally, it is involved in degradation of CREM isoform ICERIIgamma, ATF15, WEE1 kinase, and beta-catenin. CDC34 is also a target of human herpes virus 1 protein ICP0, leading to ICP0-dependent interaction with proteasomes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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