Thermo Fisher Scientific IRF8 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human IRF8. Recombinant protein control fragment (Product #RP-101887). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IRF8,uniProtId:Q02556-1,ncbiNodeId:9606,antigenRange:90-211,antigenLength:426,antigenImageFileName:PA5-82021_IRF8_Q02556-1_Rabbit.svg,antigenImageFileNamePDP:PA5-82021_IRF8_Q02556-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.30 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2789182

Product Specific Information

Immunogen sequence: PDFEEVTDRS QLDISEPYKV YRIVPEEEQK CKLGVATAGC VNEVTEMECG RSEIDELIKE PSVDDYMGMI KRSPSPPEAC RSQLLPDWWA QQPSTGVPLV TGYTTYDAHH SAFSQMVISF YY

Target Information

IRF8 (interferon regulatory factor 8) is a transcriptional activator or repressor. It specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes. IRF8 plays a negative regulatory role in cells of the immune system. It is involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells. This leads to recognition of AICE sequence, an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of the genes. Mutations in the gene can result in immunodeficiencey 32A and 32B.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.