Thermo Fisher Scientific MAP2K1 Monoclonal Antibody (OTI1E5), TrueMAB
MAP2K1(MEK1) 단백질 인식용 Mouse 단일클론 항체로 WB, IHC, ICC/IF에 사용 가능. Lyophilized 형태로 제공되며, 고순도 Affinity chromatography 정제. 인간, 마우스, 랫트, 개 반응성. 연구용으로 세포 신호전달 및 성장 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1E5 |
| Immunogen | Full-length human recombinant MAP2K1 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
MAP2K1 (MEK1) kinase is a dual-specificity protein kinase functioning in MAPK cascades, controlling cell growth and differentiation.
It is activated by growth factors, cytokines, and membrane-dependent depolarization and calcium influx.
Phosphorylation of serine residues 218 and 222 by Raf1 activates Mek1.
MAP2K1 is involved in stress response signaling, apoptosis, and cytokine-induced proliferation.
Mutations can lead to cardiofaciocutaneous syndrome.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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