Thermo Fisher Scientific MAP2K1 Monoclonal Antibody (OTI2G5), TrueMAB
MAP2K1(MEK1) 단백질을 표적하는 Mouse 단일클론 항체로, WB·IHC·ICC/IF에 사용 가능. Human, Mouse, Rat, Dog 반응성. Lyophilized 형태, 1 mg/mL 농도, PBS buffer(8% trehalose) 보존. 연구용으로 세포 성장 및 신호전달 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:400–1:4,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2G5 |
| Immunogen | Full-length human recombinant MAP2K1 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
MAP2K1 (MEK1) is a dual-specificity kinase involved in MAPK signaling pathways regulating cell growth and differentiation.
It is activated by phosphorylation at serine residues 218 and 222 by Raf1 and participates in stress response, apoptosis, and cytokine-induced proliferation.
Mutations in MAP2K1 can lead to cardiofaciocutaneous syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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