Thermo Fisher Scientific MAPK3 Monoclonal Antibody (OTI4D7), TrueMAB
MAPK3 단백질을 인식하는 고특이성 Mouse monoclonal antibody. Western blot, IHC, ICC/IF 등 다양한 응용에 적합. Lyophilized 형태로 제공되며, PBS buffer와 trehalose 포함. 연구용으로 세포 신호전달 관련 연구에 활용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4D7 |
| Immunogen | Full-length human recombinant protein of human MAPK3 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
MAPK3 (ERK1) is a serine/threonine kinase and a key activator of p90 RSK, MSK, ELK1, and Stat3, playing a crucial role in the MAPK signaling pathway. ERK1 is widely expressed and regulates meiosis, mitosis, and post-mitotic functions in differentiated cells. It is activated by various stimuli including growth factors, cytokines, viral infection, and GPCR ligands, leading to nuclear translocation and phosphorylation of nuclear targets.
Alternative splicing results in multiple ERK1 isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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