Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (ACK2), Super Bright 436, eBioscience
Mouse CD117 (c-Kit) 인식하는 ACK2 단클론 항체로, Super Bright 436 형광체 결합. Flow cytometry에 최적화되어 있으며, hematopoietic progenitor cell 분석에 적합. Violet laser(405 nm)로 여기, 436 nm에서 방출. 연구용 시약으로 비진단용.
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Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (ACK2), Super Bright 436
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 5 publications |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 8 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright™ 436, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ACK2 |
| Conjugate | Super Bright™ 436 |
| Excitation / Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2744783 |
Product Specific Information
The ACK2 monoclonal antibody reacts with mouse CD117 (c-Kit receptor), also known as Steel factor receptor or stem cell factor receptor. This 145 kDa tyrosine kinase receptor is expressed by hematopoietic progenitor cells, mast cells, and plays key roles in proliferation and differentiation.
- Reported application: Flow cytometric analysis
- Tested application: Flow cytometry on mouse bone marrow cells (≤0.25 µg/test)
- Recommended careful titration for optimal performance
- Super Bright 436: Excitation at 405 nm (violet laser), emission at 436 nm
- Recommended filter: 450/50 bandpass or equivalent
- For multiple Super Bright conjugates, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
KIT (c-KIT) is a proto-oncogene encoding a type 3 transmembrane receptor for MGF (mast cell growth factor, stem cell factor). It regulates growth and activation of hematopoietic and non-hematopoietic cells.
- Mutations in KIT are linked to gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism.
- Activation of c-Kit triggers autophosphorylation of tyrosine residues, enabling docking of Grb2 and activation of the Ras/ERK pathway.
- SCF/c-Kit signaling activates PI3K, PLC-γ, and JAK/STAT pathways, essential for hematopoiesis, stem cell maintenance, and gametogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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