Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), Super Bright 436, eBioscience
마우스 CD117(c-Kit) 인식 단클론 항체로, Super Bright 436 형광체 결합. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 0.06 µg/test로 사용 권장. 조혈모세포 및 비만세포 표지에 활용 가능. 4°C 보관, 동결 금지.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 4 publications |
| Immunocytochemistry (ICC/IF) | - | View 4 publications |
| Flow Cytometry (Flow) | 0.06 µg/test | View 61 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Pig |
| Published Species | Fish, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright™ 436, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2B8 |
| Conjugate | Super Bright™ 436 |
| Excitation/Emission Max | 413/431 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2637141 |
Additional Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395
- Brilliant UV 563
- Brilliant UV 615
- Brilliant UV 661
- Brilliant UV 737
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 605
- Brilliant Violet 650
- Brilliant Violet 786
- eFluor 450
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Super Bright 780
- Request custom conjugation
Product Specific Information
The 2B8 monoclonal antibody reacts with mouse CD117 (c-Kit receptor, Steel factor receptor, stem cell factor receptor), a 145-kDa tyrosine kinase receptor expressed on hematopoietic progenitor cells, mast cells, and others.
Interaction with steel factor promotes proliferation and differentiation of hematopoietic progenitor cells.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse bone marrow cells; recommended ≤0.06 µg/test (100 µL final volume).
Cell number: 10⁵–10⁸ cells/test (empirically determined).
Careful titration recommended.
Fluorochrome Information:
Super Bright 436 is excited by the violet laser (405 nm) and emits at 436 nm. Recommended filter: 450/50 bandpass.
Use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
KIT (c-KIT) is a proto-oncogene and type 3 transmembrane receptor for stem cell factor (SCF).
It regulates growth and activation of hematopoietic and non-hematopoietic cells.
Mutations are associated with gastrointestinal stromal tumors, mast cell disease, AML, and piebaldism.
Activation of KIT triggers downstream signaling pathways (PI3K, PLC-γ, JAK/STAT) essential for hematopoiesis, stem cell maintenance, and gametogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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