Thermo Fisher Scientific CD127 Monoclonal Antibody (A7R34), Super Bright 436, eBioscience
마우스 CD127(IL-7 receptor α chain)을 인식하는 A7R34 단클론 항체로, Super Bright 436 형광체가 결합된 제품입니다. Flow cytometry에 최적화되어 있으며, IL-7 결합 억제 및 T/B 세포 발달 연구에 활용됩니다. 4°C 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 1.0 µg/test | View 9 publications |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 436, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A7R34 |
| Conjugate | Super Bright™ 436 |
| Excitation / Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_2744785 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 561
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 661
- Brilliant UV 737
- Brilliant UV 805
- Brilliant Violet 421
- Brilliant Violet 650
- Brilliant Violet 711
- eFluor 450
- eFluor 660
- FITC
- Functional Grade
- NovaFluor Red 700
- NovaFluor Red 710
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- PerCP-eFluor 710
- Super Bright 645
- Request custom conjugation
Product Specific Information
The A7R34 monoclonal antibody reacts with mouse CD127, the high-affinity alpha subunit of the IL-7 receptor. CD127 is expressed by immature B cells in the bone marrow, double-negative (CD4⁻CD8⁻), single-positive (CD4⁺ and CD8⁺), but not double-positive thymocytes. Mature peripheral T cells express CD127 at low levels.
A7R34 inhibits IL-7 binding to its receptor and is used in vivo and in vitro to study IL-7 roles in T and B cell development and activation. It also blocks binding of SB/199, another CD127-recognizing antibody.
Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of mouse splenocytes, ≤1 µg/test (100 µL final volume).
Super Bright 436 is excited by a violet laser (405 nm) and emits at 436 nm. Recommended filter: 450/50 bandpass or equivalent. Ensure instrument compatibility.
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific interactions.
Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD127 (Interleukin-7 receptor alpha chain, IL7Rα) regulates lymphopoiesis. The active IL7R receptor is an alpha/gamma chain heterodimer.
- The gamma(c) chain activates signal transduction.
- The alpha chain determines specific signaling events.
CD127 promotes proliferation of precursor B cells, thymocytes, and mature CD4⁺/CD8⁺ T cells.
Dysfunction is associated with severe combined immunodeficiency (SCID) and T⁻/B⁻/NK⁺ SCID variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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