Thermo Fisher Scientific DARPP-32 Recombinant Rabbit Monoclonal Antibody (SU0329)
DARPP-32 단백질을 인식하는 재조합 토끼 단클론 항체로, 높은 특이성과 민감도를 제공. Western blot, ICC/IF, Flow cytometry 등에 사용 가능. HEK293 발현 시스템 기반, Protein A 정제, 액상 형태. 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000–1:5,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:50–1:200
- View 1 publication
Flow Cytometry (Flow)
- Tested Dilution: 1:50–1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SU0329 |
| Immunogen | Recombinant protein within Human DARPP-32 (aa 1–94) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809405 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
DARPP-32 is a dopamine (DA) and AMP-regulated ~32 kDa phosphoprotein associated with dopaminoceptive neurons.
- When phosphorylated on Thr34, it inhibits Protein Phosphatase I.
- When phosphorylated on Thr75, it acts as an inhibitor of PKA.
Phosphorylation of DARPP-32 plays a critical role in dopaminergic neurotransmission and is implicated in the actions of alcohol, caffeine, and Prozac®.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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