Thermo Fisher Scientific FAK Recombinant Rabbit Monoclonal Antibody (5H18L19)
FAK 단백질을 검출하기 위한 Thermo Fisher Scientific의 재조합 토끼 단클론 항체. Western blot, IHC, ICC/IF에 적용 가능. 높은 특이성과 재현성을 제공하며, 동물 유래 성분이 없는 포뮬레이션으로 일관된 성능을 보장.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–3 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | 2–4 µg | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 5H18L19 |
| Immunogen | Recombinant protein corresponding to amino acids 853–1052 of human FAK1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2532384 |
Product Specific Information
- This antibody is predicted to react with mouse based on sequence homology.
- Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generates a ~25 kDa light chain band and a ~50 kDa heavy chain.
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select the best candidates for production.
- Advantages include improved specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity due to the larger rabbit immune repertoire.
Target Information
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase acting as a substrate for Src and a key element in integrin signaling.
FAK plays a crucial role in cell spreading, differentiation, migration, cell death, and the G1–S phase transition of the cell cycle.
It localizes to focal adhesions—sites where cells attach to the extracellular matrix via integrin receptors.
Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin, promoting association with Grb2 and c-Src PTK, and activation of ERK2 MAP kinase.
Multiple transcript variants exist for FAK, with several full-length isoforms identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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