Thermo Fisher Scientific NUP98 Monoclonal Antibody (J.750.3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:400-1:1600

-

Immunoprecipitation (IP)

1:50

-

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

J.750.3

Immunogen

Synthetic peptide corresponding to residues surrounding Pro671 of human NUP98 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NUP98,uniProtId:P52948-1,ncbiNodeId:9606,antigenRange:671,antigenLength:1817,antigenImageFileName:MA5-14907_NUP98_P52948-1_Rabbit.svg,antigenImageFileNamePDP:MA5-14907_NUP98_P52948-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

18 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_11006366

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Signal-mediated nuclear import and export proceed through the nuclear pore complex (NPC), which is comprised of approximately 50 unique proteins collectively known as nucleoporins. The 98 kDa nucleoporin is generated through a biogenesis pathway that involves synthesis and proteolytic cleavage of a 186 kDa precursor protein. This cleavage results in the 98 kDa nucleoporin as well as a 96 kDa nucleoporin, both of which are localized to the nucleoplasmic side of the NPC. Rat studies show that the 98 kDa nucleoporin functions as one of several docking site nucleoporins of transport substrates. The human gene has been shown to fuse to several genes following chromosome translocations in acute myelogenous leukemia (AML) and T-cell acute lymphocytic leukemia (T-ALL). This gene is one of several genes located in the imprinted gene domain of 11p15. 5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.