Thermo Fisher Scientific Osteopontin Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

By Western Blot, a doublet band of approximately 65 kDa and a band of 30 kDa is observed due to glycosylation.
A suggested positive control is 293 cells.
The Neutralization Dose50 (ND50) for anti-mouse osteopontin is approximately 1–3 µg/mL in the presence of 2 µg/mL recombinant mouse osteopontin using 293 cells.

Target Information

Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors.
It is a multifunctional protein involved in bone mineralization, cell adhesion, migration, chronic inflammatory disease, and transformation.
Proteolytic cleavage by thrombin and matrix metalloproteinases near the integrin-binding Arg-Gly-Asp sequence modulates OPN function and integrin binding.
Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors, providing a molecular niche with survival advantage and serving as substrates for plasmin and cathepsin D.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.