Thermo Fisher Scientific Osteopontin Polyclonal Antibody
Thermo Fisher Scientific의 Osteopontin Polyclonal Antibody는 마우스 Osteopontin을 인식하는 goat IgG 항체입니다. WB, IHC, ELISA 등 다양한 응용에 적합하며, 고순도 친화 크로마토그래피로 정제되었습니다. 4°C 단기, -20°C 장기 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| Immunohistochemistry (IHC) | 5–15 µg/mL | View 3 publications |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| ELISA | 0.2–0.8 µg/mL | - |
| Neutralization (Neu) | 1–3 µg/mL | View 1 publication |
| Inhibition Assays (IA) | 1–3 µg/mL | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant full-length mouse protein expressed in NSO cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term; for long-term storage, keep at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_795087 |
Product Specific Information
By Western Blot, a doublet band of approximately 65 kDa and a band of 30 kDa is observed due to glycosylation.
A suggested positive control is 293 cells.
The Neutralization Dose50 (ND50) for anti-mouse osteopontin is approximately 1–3 µg/mL in the presence of 2 µg/mL recombinant mouse osteopontin using 293 cells.
Target Information
Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors.
It is a multifunctional protein involved in bone mineralization, cell adhesion, migration, chronic inflammatory disease, and transformation.
Proteolytic cleavage by thrombin and matrix metalloproteinases near the integrin-binding Arg-Gly-Asp sequence modulates OPN function and integrin binding.
Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors, providing a molecular niche with survival advantage and serving as substrates for plasmin and cathepsin D.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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