Thermo Fisher Scientific Catenin alpha-1 Polyclonal Antibody
Catenin alpha-1 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체로, WB, IHC, ICC/IF에 적용 가능. 인간, 생쥐, 개, 쥐, Xenopus 반응성. 세포 부착 복합체 연구 및 카데린-액틴 상호작용 분석에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunohistochemistry (IHC) | - | 2 publications |
| Immunocytochemistry (ICC/IF) | 1:200 | 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat, Xenopus |
| Published Species | Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide conjugated to KLH, corresponding to residues E(873) K Q D E T Q T K I K R A S Q(887) of human alpha 1 Catenin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | IgG fraction |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_779553 |
Product Specific Information
- Recommended positive controls: A431 and cytosolic fraction of rat embryonic brain, MDCK, MCF-7.
- PA1-25081 is expected to cross-react with mouse (100% conserved) and Xenopus laevis (100% conserved) due to sequence homology.
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
Catenin alpha-1 associates with the cytoplasmic domain of various cadherins. The catenin–cadherin complex is linked to the actin filament network, playing a critical role in cell adhesion. It can associate with both E- and N-cadherins.
Originally thought to mediate linkage of cadherins to the actin cytoskeleton at adherens junctions, later studies suggest it does not bind directly to F-actin within the complex, implying a different linkage mechanism.
The homodimeric form may regulate actin filament assembly and inhibit branching by competing with the Arp2/3 complex for actin binding. It plays an important role in cell differentiation.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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