Thermo Fisher Scientific Zyxin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

-

Immunohistochemistry (IHC)

-

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Immunocytochemistry (ICC/IF)

1:100

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Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide, conjugated to KLH at an N-terminal added cysteine, corresponding to residues 134-150 of human Zyxin. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Zyxin,uniProtId:Q15942-1,ncbiNodeId:9606,antigenRange:134-150,antigenLength:572,antigenImageFileName:PA1-25162_Zyxin_Q15942-1_Rabbit.svg,antigenImageFileNamePDP:PA1-25162_Zyxin_Q15942-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

12.7 mg/mL

Purification

IgG fraction

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2221183

Product Specific Information

Recommended positive controls: HeLa, Rat1.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Zyxin is involved in focal adhesions, which are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.